期刊论文

Based on metabolites of biosynthetic pathway to research on whether day lily(Hemerocallis citrine Baroni)contains colchicine

中文

湖南农业大学学报(自然科学版)

1007-1032

2017

43

5

485-489

10.13331/j.cnki.jhau.2017.05.004

国家科技支撑计划项目

本校为第一机构

基于已解析的秋水仙中秋水仙碱生源合成途径,采用高效液相色谱-四级杆/飞行时间质谱联用（HPLC-Q/TOF MS）方法,检测黄花菜和秋水仙样本中秋水仙碱生源合成途径中的中间代谢产物（以下简称中间代谢物）。结果从黄花菜样本中未检出中间代谢物和终产物秋水仙碱,而在秋水仙样本中均能检出中间代谢物和秋水仙碱,其中的中间代谢物秋水仙胺（Cp5）、去乙酰基秋水仙碱（Cp6）和终产物秋水仙碱（Cp7）已进一步经对照品确证,因此认为食用黄花菜中不存在秋水仙碱的生源合成途径,也不含秋水仙碱。

Based on parsed colchicine biosynthetic pathway in Colchicum autumnale L.(C.autumnale), the intermediate metabolites of colchicine biosynthetic pathway (IMCBP) were analyzed by HPLC–Q/TOF MS in Hemerocallis citrina Baroni (H. citrina) and C.autumnale samples. The result showed that IMCBP was found in C.autumnale but not detected in H. citrine samples. Demecolcine (Cp5),deacetylcolchicine(Cp6) and colchicine(Cp7)were confirmed by authentic standard substances. The results support that there are no co...