期刊论文

Inhibitory effects of tea extracts EGCG,GCG and ECG on the melanogenesis in melanoma cell B16

中文

湖南农业大学学报(自然科学版)

1007-1032

2017

43

4

405-410

10.13331/j.cnki.jhau.2017.04.011

国家自然科学基金项目（31100502）； 国家现代农业茶叶产业技术体系项目（CARS–23）；

本校为第一机构

为研究茶叶提取物表没食子儿茶素没食子酸酯(epigallocatechin gallate, EGCG)、没食子儿茶素没食子酸酯(gallocatechingallate, GCG)和表儿茶素没食子酸酯(epicatechingallate, ECG)对体外培养B16小鼠黑色素瘤细胞黑色素生成及酪氨酸酶活性的影响,分别用不同浓度的EGCG、GCG、ECG和熊果苷(Arbutin,AR)处理细胞,观察效应物对细胞形态的影响,用溴化二苯四偶氮法(MTT法)测定茶叶提取物对细胞增殖率的影响,以左旋多巴(L–DOPA)为底物,测定细胞内酪氨酸酶的活性,采用氢氧化钠裂解法测定细胞内黑色素的含量。结果显示,EGCG、 ECG、GCG能显著抑制B16细胞的黑色素生成和酪氨酸酶的活性,且呈剂量依赖关系...

Inhibitory effects of tea extracts EGCG, GCG and ECG on melanoma cell B16 from mouse were studied in this paper. Cells were treated with different concentrations of GCG, ECG, EGCG and Arbutin, and their morphology was observed in an inverted microscope. The proliferation rate of B16 was determined using MTT method, tyrosinase activity was detected by using L-DOPA as substrate, and NaOH-lysis approach was employed to measure the melanin synthesis. The results showed that EGCG, ECG, GCG could significantly inhibit the melanogenesis and tyrosinase...