期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2017

43

2

135-139

10.13331/j.cnki.jhau.2017.02.004

2013GA780085:国家级星火计划 2013B010404047:广东省科技计划 2014A030307023,2016 A030313669:广东省自然科学基金 2014A01012,2014A02012:湛江市科技攻关计划

本校为第一机构

采用PMA和ox–LDL构建THP–1巨噬细胞源性泡沫细胞模型,观察不同浓度(5、10、15 nmol/L)仙人掌多糖(ODP–Ia)对泡沫细胞内脂质、胆固醇蓄积以及介导胆固醇流出的ABCA1、ABCG1、SR–BI基因表达的影响。结果显示:THP–1巨噬细胞泡沫化后胞内蓄积了大量脂质,高剂量(15 nmol/L)ODP–Ia能够显著减少胞内脂质、胆固醇蓄积,并增强ABCA1、ABCG1、SR–BI mRNA及蛋白表达(与单独ox–LDL处理组比较,P

In this study, THP–1 cells were induced into macrophages and THP–1 macrophage–derived foam cell by phorbolmyristate acetate (PMA) and oxidized low–density lipoprotein (ox–LDL), and then treated with different concentrations(5, 10, 15 nmol/L) of ODP–Ⅰa, to investigate the beneficial effects of ODP–Ⅰa on the cholesterol accumulation in the THP–1 macrophage derived foam cell. Meanwhile, the mRNA expression and protein levels of ATP binding cassette transporter ABCA1, ATP binding cassette transporter ABCG1 and scavenger receptor class B t...