期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2017

43

4

366-371

10.13331/j.cnki.jhau.2017.04.004

湖南省教育厅高校重点实验室开放项目（15K060）；

本校为第一机构

为了探明十字花科植物不同物种PIN3基因之间的进化差异,运用Brassica Database数据库及十字花科基因数据库在线平台,对拟南芥(Arabidopsis thaliala)、红花荠菜(Capsella rubella)、芜青(Brassica rapa)、油菜(Brassica napus)、甘蓝(Brassica oleracea)等17个已完成基因组测序的十字花科物种的18个(油菜含有2个)PIN3同源性基因进行分析,并对其推导的编码蛋白(PIN3)进行了系统进化分析。结果表明,18个PIN3序列间的相似性为86.74%,PIN3推导蛋白之间的相似度为90.93%,蛋白等电点约为7.15~9.44,芜青PIN3等电点明显偏高。 PIN3的氨基酸比对分析结果表明,在多个糖基化位点出现具有种属差异性和符合进...

In order to explore the evolutionary differences between PIN3 genes in different species of Brassicaceae, the Brassica database and the Brassicaceae gene database were used. In this paper 18 PIN3 genes were identified by sequences blast in the database of 17 sequenced Brassicaceae species (there were two homologous PIN3 in Brassica napus). The phylogenetics of the genes and their predicted proteins (PIN3) were carried out by bioinformatics analysis. The results indicated that the similarity of 18 PIN3 gene sequences in the Brassicaceae species was 86.74%, while the proteins similarity was 90.9...