期刊论文

Genetic diversity and geographical range expansion in Hunan populations of Lissorhoptrus oryzophilus

中文

湖南农业大学学报(自然科学版)

1007-1032

2018

44

6

613-619

10.13331/j.cnki.jhau.2018.06.007

长沙市科技局重点项目(kc1701003).

本校为第一机构

为全面了解湖南省稻水象甲的入侵扩散特点,运用简单重复序列间区(ISSR)分子标记方法及对比COⅠ基因、ITS2片段的序列信息,探讨全省17个地理种群的遗传多样性和扩散模式。结果表明:湖南省稻水象甲种群COⅠ基因、ITS2片段的序列非常保守,不能提供有效的多态性位点信息,而ISSR的多态性信息非常丰富,其多态性位点百分率可达93.24%;基于ISSR的数据分析发现,湖南省稻水象甲的Nei’s基因多样性指数为0.363 0,各地理种群间基因分化系数为0.414 3,种群之间的遗传距离与地理距离无相关性。湖南省稻水象甲地理种群分化不符合地理隔离模式,以人为因素导致的扩散为主,自然扩散为辅。

To understand the geographical range expansion of Lissorhoptrus oryzophilus (L. oryzophilus), a rice weevil,in Hunan province, inter–simple sequence repeat (ISSR) molecular markers, mitochondrial COⅠgene and ITS2 sequence were examined to investigate the genetic diversity and expansion model of 17 geographic populations. The results showed that mitochondrial COⅠ gene and ITS2 sequence were both conservative in different individuals, which was inapropriate for effective polymophism analysis. However, the percentage of polymorphic bands of ISS...