期刊论文

Establishment of Artemisia annua L. suspension cultured cell lines and its genetic transformation

中文

湖南农业大学学报(自然科学版)

1007-1032

2018

44

6

607-612

10.13331/j.cnki.jhau.2018.06.006

湖南省大学生科技创新项目(DFCXS201206).

本校为第一机构

为建立稳定的黄花蒿悬浮细胞系,以黄花蒿428–A植株的子叶、茎、腋芽和叶片为外植体,以MS为基础培养基,采用3因素(6–BA、NAA、2,4–D)4水平的L_(16)(4~3)正交试验,优化黄花蒿愈伤组织诱导培养基,分析不同质量浓度(0、5、10、15、20、25、30 mg/L)潮霉素对黄花蒿悬浮细胞生长的影响,比较羧苄青霉素(0、100、 150、200、250、300 mg/L)对农杆菌的抑制效果,并运用农杆菌介导法对黄花蒿悬浮细胞进行GUS报告基因及色氨酸单加氧酶iaaM基因的遗传转化。结果表明:黄花蒿叶片愈伤诱导率较高,叶片愈伤组织较优培养基为MS+6–BA 1.0 mg/L+NAA 0.5 mg/L,出愈率可达99.33%;潮霉素最适筛选质量浓度为30 mg/L,...

To establish cultured cell lines of Artemisia annua L., we used its leaves and stems as explants, MS–based medium and performed three factors of 6–BA, NAA, 2,4–D mass concentration with each factor of 4 levels using L_(16)(4~3) orthogonal table for orthogonal test. We also optimized different concentrations of hygromycin and carbenicillin on the growth of the suspension cells and the inhibition effect on Agrobacterium tumefaciens and applied Agrobacterium– mediated method to do genetic transformation of GUS reporter gene and tryptophan mono...