期刊论文

The genetic effect analysis of iaaM specific expressed in trichome in tobacco and Arabidopsis

中文

湖南农业大学学报(自然科学版)

1007-1032

2018

44

3

284-289

10.13331/j.cnki.jhau.2018.03.0010

15K060:教育厅项目 DFCXY201610:湖南农业大学东方科技学院大学生创新项目

本校为第一机构

利用色氨酸单加氧酶基因iaaM与拟南芥表皮毛特异表达GL2基因启动子重组后,构建了在植物表皮毛细胞中特异表达iaaM基因的重组基因载体;采用根癌农杆菌叶盘转化法将重组基因转化到烟草WS38(对照)中,筛选和鉴定出了6株转化烟草植株;将该重组基因以根癌农杆菌花序浸渍法转至拟南芥(Colombia ecotype,对照),筛选出9株拟南芥转化植株,并对稳定转化的转基因植株表皮毛进行观察。结果表明:转基因烟草表皮毛较对照植株显著增长,其长度最长为对照的2.2倍,但转基因拟南芥表皮毛长度与对照间无显著差异。对烟草幼叶进行生长素浓度检测,转iaaM基因烟草吲哚乙酸含量显著提高,说明iaaM在烟草表皮毛细胞中表达,使...

The iaaM, a tryptophan monooxygenase gene deriving from bacteria, can catalyze the synthesize of indole acetic acid (IAA) with tryptophan via indoleacetamide pathway in plants. A trichome specific expression of iaaM vector by recombination of the trichome specific GL2 gene promoter of Arabidopsis and iaaM gene coding region was constructed. The recombinant was transformed into tobacco WS38 by Agrobacterium tumefaciens mediated leaf disc transformation and 6 transformed tobacco plantlets were screened and identified. A comparative experiment was transformation of Arabipdopsis by the Agrobacteri...