期刊论文

Effects of drought stress on nitrogen content and expression of nitrogen metabolism related genes in Poncirus trifoliata (L.) Raf

中文

湖南农业大学学报(自然科学版)

1007-1032

2018

44

5

500-505,538

10.13331/j.cnki.jhau.2018.05.009

现代农业（柑橘）产业技术体系建设专项（CARS–026）；

本校为第一机构

以枳为试验材料,进行干旱胁迫处理(对照组的基质相对持水量维持在55%~65%),分别在处理后第7、 17、27、37天采样,研究干旱胁迫对枳侧根、主根、茎、叶片中全氮含量的影响以及氮代谢途径中NRT、NR、 NiR、GS、GOGAT、GDH等16个相关基因在侧根和叶片中的表达。结果表明:随着干旱时间的延长,枳的侧根、主根、茎、叶中全氮含量与对照相比均显著下降,其中侧根全氮含量下降最为显著;枳叶片中与氮代谢相关的16个基因均能被干旱胁迫诱导而上调表达,且多数基因在干旱第27天上调至最高水平,而后随着叶片的萎蔫而下调;枳侧根中有8个基因(NRT1.2、NRT1.7、GS2、NADH-GOGAT2、NADP-GDH、GDH1、GDH2、GDH3)在干...

The experiment of drought treatment of rootstock Poncirus trifoliata (L.) Raf. was carried out by using the method of drought stress and water control (the relative water holding capacity of the control group was maintained at 55% to 65%) on potted. The effects of drought stress on the total nitrogen content of the lateral roots, main roots, stems and leaves of P. trifoliata, as well as the expression of 16 genes related to NRT, NR, NiR, GS, GOGAT and GDH in the lateral roots and leaves of the nitrogen metabolism pathway were analyzed on 7 days, 17 days, 27 days and 37 days after treatment. Th...