期刊论文

Influence of gossypol on oxidative stress and apoptosis in piglet sertoli cells

中文

湖南农业大学学报(自然科学版)

1007-1032

2019

45

3

312-315

10.13331/j.cnki.jhau.2019.03.016

湖南省教育厅优秀青年项目（17B125）；

本校为其他机构

以原代分离培养仔猪睾丸支持细胞为模型,经含不同浓度(0、2.5、5、10、20、40、80、160 μmol/L)乙酸棉酚或40 μmol/L乙酸棉酚加800 μmol/L的乙酰半胱氨酸(NAC)的培养基培养24 h后,检测细胞增殖率、细胞内活性氧(ROS)水平和丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性、培养基中乳酸脱氢酶(LDH)活性及细胞凋亡情况,研究棉酚对仔猪睾丸支持细胞的氧化应激及细胞凋亡的影响。结果显示:棉酚呈浓度依赖性抑制仔猪睾丸支持细胞增殖率,棉酚浓度大于等于10 μmol/L时,细胞增殖率极显著降低;棉酚造成细胞内ROS水平显著升高、SOD活性显著降低、MDA含量升高及培养基中LDH活性升高;NAC显著抑制棉酚诱导的细...

Using primary cultured piglet sertoli cells as cell model,different concentration of gossypol(0,2.5,5,10,20,40,80,160 μmol/L) or 40 μmol/L gossypol plus 800 μmol/L acetylcysteine(NAC) for 24 h were applied in the study.The cell proliferation,intracellular level of reactive oxygen species(ROS),malondialdehyde(MDA) and supper oxide dismutase(SOD) activity,lactate dehydrogenase(LDH) activity in culture medium and cell apoptosis were measured.The results showed that the cell proliferation of piglet sertoli cells was significantly inhibited by go...