期刊论文

Characterization of protein components and toxicity of Deinagkistrodon acutus venom in Yongzhou of Hunan

中文

湖南农业大学学报(自然科学版)

1007-1032

2019

45

5

541-547

10.13331/j.cnki.jhau.2019.05.016

本校为第一机构

采用Nano–LC–ESI–MS/MS蛋白鉴定技术对湖南永州尖吻蝮蛇蛇毒蛋白组分进行质谱分析,应用常规体外试管法对尖吻蝮蛇蛇毒体外溶血值进行检测,采用改良寇式法对尖吻蝮蛇蛇毒LD_(50)进行测定。结果显示:尖吻蝮蛇蛇毒含有50种蛋白,蛋白相对分子质量集中在1.0×10~4~2.0×10~4(46.9%)、4.0×10~4~5.0×10~4(22.4%)、 2.0×10~4~3.0×10~4(10.6%)和7.0×10~4~8.0×10~4(7.6%),其中代表性的高丰度蛋白有蛇毒金属蛋白酶A(11.7%)、蛇毒金属蛋白酶H1(9.8%)、蕲蛇类凝血酶-2(7.3%)、抗凝血酶A-A亚基(6.8%),低丰度蛋白(

The protein components of the venom obtained from Deinagkistrodon acutus in Yongzhou area of Hunan were identified and characterized using Nano-LC-ESI-MS/MS. The in vitro hemolysis of D. acutus venom was conducted with use of the conventional test tube method, and the LD_(50) of the venom was measured using a challenge test in a KM mouse model. Fifty types of proteins were identified, and the protein relative molecular mass are mainly distributed in 1.0×10~4~ 2.0×10~4(46.9%), 4.0×10~4~5.0×10~4(22.4%), 2.0×10~4~3.0×10~4(10.6%) and 7.0×10~...