期刊论文

The purification process and antioxidant activity of polyphenols from the pericarpium trichosanthis

中文

湖南农业大学学报(自然科学版)

1007-1032

2019

45

4

420-424

10.13331/j.cnki.jhau.2019.04.014

湖南省重大科技成果转化项目（2014CK1005）；

本校为第一机构

以瓜蒌皮为研究对象,采用大孔吸附树脂法纯化瓜蒌皮中的多酚类化合物,对纯化工艺参数进行筛选,对纯化产物的抗氧化活性进行检测。结果表明:AB–8大孔吸附树脂对瓜蒌皮多酚具有良好的吸附和解吸性能,最佳吸附工艺参数为提取液中多酚质量浓度0.60 mg/mL,料液pH值3.0,上样流速2 BV/h,上样体积4 BV;最佳洗脱工艺参数为洗脱液乙醇的体积分数60%,体积6 BV,洗脱流速2 BV/h;纯化后产品中多酚的含量达49.52%,较纯化前提高57倍;瓜蒌皮多酚纯化前后对DPPH+自由基清除作用的IC_(50)分别为2.01 mg/mL和0.53 mg/mL,对ABTS+自由基清除作用的IC_(50)分别为0.62 mg/mL和0.13 mg/mL,纯化后抗氧化活性增强了4~5倍。...

In this study, we investigated the variables of purification process of polyphenolic compounds from pericarpium trichosanthis and these variable effects on the antioxidation activity of the purified products. The method to purify was macroporous adsorption resin. The results showed that AB-8 macroporous resin has the best adsorption and desorption performance. The optimized adsorption process variables should be at: the concentration of polyphenols of extract 0.6 mg/mL; pH 3.0, the flow rate of fluid 2 BV/h, the volume 4 BV. The optimized elution parameters included the eluting regent 60 % eth...