期刊论文

Bioinformatics and expression analysis of polyphenol oxidase gene family in potato

中文

湖南农业大学学报(自然科学版)

1007-1032

2019

45

6

601-610

10.13331/j.cnki.jhau.2019.06.007

201503001:公益性行业(农业)科研专项 2017A020208033:广东省省级科技计划 2017B020201005:广东省省级科技计划 2017B020232002:广东省省级科技计划 2018A030313336:广东省自然科学基金 :省级农业发展 :农村工作专项 CAAS-ASTIP-IVFCAAS:科技创新项目 18KFXM03:国家重点实验室基金

本校为第一机构

通过搜索马铃薯全基因组,获得马铃薯多酚氧化酶(PPO)基因家族序列,并对其进行生物信息学及表达分析。结果显示:共获得12个马铃薯PPO基因,即StuPPO1、StuPPO2、StuPPO3、⋯、StuPPO12;除StuPPO9位于2号染色体外,其余11个基因串联于8号染色体的276 kb范围内;对具有3个典型结构域(Tyrosinase、 PPO1_DWL、PPO1_KFDV)的基因蛋白序列进行进一步分析,结果显示StuPPO1、StuPPO2、StuPPO3、StuPPO4、 StuPPO6蛋白序列相似性大于80%,且大部分StuPPO蛋白位于叶绿体;启动子顺式作用元件分析显示,StuPPO1、 StuPPO2、StuPPO3、StuPPO6、StuPPO8、StuPPO9具有MeJA响应元件,StuPPO1、StuPPO3、StuPPO4、StuPPO...

The sequences of the polyphenol oxidase(PPO) gene family were searched from potato genome and were subject to bioinformatics and expression analysis. The results showed that a total of 12 PPO genes, StuPPO1, StuPPO2, StuPPO3, ⋯, StuPPO12 with exception of StuPPO9 localized on chromosome 2, were sorted out and all of them located in the 276 kb range of chromosome 8. Further analysis of the PPO protein sequences with 3 typical structural domains (Tyrosinase, PPO1_DWL, PPO1_KFDV) showed that the similarity of StuPPO1, StuPPO2, StuPPO3, StuPPO4 an...