期刊论文

Effects of SSCD1 mutation on phenylalanine-induced anthocyanin accumulation in Arabidopsis thaliana

中文

湖南农业大学学报(自然科学版)

1007-1032

2020

46

1

33-37

10.13331/j.cnki.jhau.2020.01.006

2014CB160308:国家重点基础研究发展计划（973计划）

本校为第一机构

为探索花青素生物合成的调控机制,以模式植物拟南芥野生型Col–0和酪氨酸降解途径缺陷突变体sscd1为试验材料,分析5种浓度(0、0.1、0.5、1.0、2.0 mmol/L)外源苯丙氨酸处理后花青素的积累和花青素生物合成相关基因的表达,探讨酪氨酸降解途径受阻是否影响苯丙氨酸诱导花青素的积累。结果发现:外源添加不同浓度苯丙氨酸能提高拟南芥幼苗花青素的含量,而且花青素的含量随着苯丙氨酸浓度的增加而增多;苯丙氨酸处理后, sscd1突变体幼苗中花青素的积累增多,同时花青素生物合成基因,如PAL、CHI、CHS、DFR、LDOX、UF3GT的表达水平在sscd1突变体中都显著上调,表明SSCD1基因突变会阻断酸酪氨酸降解,增加苯...

In this paper, the model plant Arabidopsis thaliana wild type Col-0 and tyrosine degradation pathway defective mutant sscd1 were used as experimental materials to explore the regulatory mechanism of anthocyanin biosynthesis, to analyze the accumulation of anthocyanin and anthocyanin biosynthesis related gene expression after treatment with exogenous phenylalanine, and to explore whether the blocked tyrosine degradation pathway affect phenylalanine-induced anthocyanin accumulation. The results showed that the accumulation of anthocyanins in sscd1 mutant seedlings increased after phenylalanine t...