期刊论文

Relaxed effect of Gelsemium elegans Benth extraction by ethanol on intestinal smooth muscle of rabbit in vitro and its mechanism

中文

湖南农业大学学报(自然科学版)

1007-1032

2020

46

4

461-465

10.13331/j.cnki.jhau.2020.04.013

国家重点研发计划专项（2017YFD0501403）； 国家自然科学基金项目（31572563）；

本校为第一机构

采用BL–420E生物机能实验系统,观察钩吻醇提物对兔离体肠肌自主收缩及对工具药乙酰胆碱(Ach)、 BaCl2和组胺(HA)所致痉挛性收缩的影响,并探讨肌浆网Ryanodine受体阻断剂钌红(RR)、三磷酸肌醇(IP3)受体阻断剂肝素钠(HP)和一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(L–NAME)对钩吻醇提物作用的影响。结果表明:终质量浓度0.16~0.64 mg/mL钩吻醇提物可剂量依赖性抑制离体肠肌自主收缩频率和张力;终质量浓度0.32 mg/mL钩吻醇提物对BaCl_2、HA诱导的离体肠肌收缩频率和张力具有显著抑制作用(P

In this experiment, the BL-420E biological function experiment system was used to investigate the effect of Gelsemium elegans Benth extraction by ethanol(GEBEE) on the independent contraction of isolated intestinal muscles in rabbits and the spasmodic contraction induced by Ach, BaCl_2 and HA, and to explore the effect of sarcoplasmic reticulum Ryanodine receptor blocker RR, IP_3 receptor blocker HP and nitric oxide synthase inhibitor L-NAME on GEBEE. The results showed that 0.16-0.64 mg/mL of GEBEE could inhibit the voluntary contraction frequency and tension of isolated rabbit intestinal mus...