期刊论文

Pulsed electric field extraction of flavonoids from Exocarpium Citri grandis and its antioxidant activity detection

中文

湖南农业大学学报(自然科学版)

1007-1032

2021

47

3

305-311

10.13331/j.cnki.jhau.2021.03.009

31671858:国家自然科学基金 2019JJ40114:湖南省自然科学基金 kq1801016:长沙市科技计划

本校为第一机构

采用单因素试验考察脉冲次数、料液比、电场强度对高压脉冲电场(PEF)辅助处理提取水性介质中化橘红黄酮得率的影响,通过响应面分析法优化化橘红黄酮的PEF提取工艺;利用乙酸乙酯萃取和AB-8大孔树脂吸附方法纯化提取的化橘红黄酮;采用1,1-二苯基-2-三硝基苯肼(DPPH)法、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)法和铁离子还原测定(FRAP)法对化橘红黄酮进行体外抗氧化活性研究。结果表明:高压脉冲电场提取化橘红黄酮的最优工艺为脉冲次数250、电场强度7.8 kV/cm和料(W,g)液(V,mL)比1∶70,此时黄酮得率为30.34 mg/g;大孔树脂吸附、乙酸乙酯萃取的黄酮质量分数分别为51.20%、20.90%,大孔树脂吸附...

In this study, single-factor experiments were used to explore the effects of pulse number, material-to-liquid ratio, and field strength on the yield of flavonoids extracted from Exocarpium Citri grandis in aqueous medium with high-voltage pulsed electric field auxiliary extraction technology and the optimum extraction conditions were sorted out by a response surface methodology. The flavonoids were purified by ethyl acetate extraction and AB-8 macroporous resin adsorption method. Meanwhile, the anti-oxidant activity in vitro was studied through...