期刊论文

Effect of tryptophan on cell death of Arabidopsis thaliana tyrosine degradation pathway defective mutant sscd1

中文

湖南农业大学学报(自然科学版)

1007-1032

2021

47

4

424-428

10.13331/j.cnki.jhau.2021.04.009

2014CB160308:国家重点基础研究发展计划（973计划）

本校为第一机构

为了解色氨酸处理是否影响sscd1的细胞死亡,以拟南芥野生型Columbia(Col–0)和sscd1突变体为试验材料,用色氨酸处理,观察并统计幼苗的死亡情况,检测叶绿素含量,分析酪氨酸降解途径基因HGO、MAAI和SSCD1以及活性氧诱导基因BAP1、OXI1、ZP的表达。结果表明:色氨酸处理能明显抑制sscd1突变体幼苗死亡,增加叶绿素的合成,抑制sscd1突变体中酪氨酸降解途径基因HGO和MAAI以及活性氧诱导基因BAP1、OXI1、 ZP的上调。推测色氨酸可能通过增加叶绿素的生物合成,减少活性氧的产生来抑制酪氨酸降解途径突变体sscd1的细胞死亡。

To understand whether tryptophan treatment affects the cell death of sscd1, we treated Arabidopsis thaliana wild type Columbia(Col-0) and tyrosine degradation pathway defect sscd1 mutant with tryptophan. We observed the phenotypes of seedlings, detected chlorophyll content and analyzed the expression of tyrosine degradation pathway genes and reactive oxygen species(ROS) inducible genes. The results showed that tryptophan treatment could significantly inhibit the death of sscd1 mutant seedlings, increase chlorophyll biosynthesis, and inhibit the up-regulation of tyrosine degradation pathway gen...