期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2022

48

1

87-94,119

10.13331/j.cnki.jhau.2022.01.014

湖南省自然科学基金科教联合项目（2018JJ5050）； 湖南生物机电职业技术学院院级项目（20TD06）；

本校为其他机构

为了解湘西黄牛粪源大肠埃希菌的耐药情况,从湘西黄牛主产区花垣县和凤凰县6个不同规模的湘西黄牛养殖场采集黄牛肛门拭子样品,用麦康凯培养基和大肠埃希菌特异性引物分离、鉴定大肠埃希菌;采用K-B法对分离的大肠埃希菌进行5类共18种常用抗菌药物的敏感性试验;利用超高通量荧光定量PCR对16株多重耐药大肠埃希菌的7类共22种耐药基因进行检测;用大肠埃希菌8个管家基因对此16株分离株进行多位点序列分型(MLST),并运用goeBURST程序和MEGA 7.0对其进行ST聚类分析和进化树分析。结果表明:从采集的206份黄牛肛门拭子样品中共分离、鉴定出大肠埃希菌170株;分离自各牛场的大肠埃希菌对四环素、多西环素、...

In order to understand the drug resistance of fecal Escherichia coli from Xiangxi cattle,anal swab samples of cattle were collected from six Xiangxi cattle farms of different scales in Huayuan County and Fenghuang County,the main production areas of Xiangxi cattle,and Escherichia coli was isolated and identified by maconkey medium and Escherichia coli specific primers;the sensitivity test of 18 kinds of common antibiotics in 5 categories was carried out on the isolated Escherichia coli by K-B method;22 drug resistance genes of 7 classes of 16 strains of multidrug resistant Escherichia coli wer...