期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2022

48

2

190-195

10.13331/j.cnki.jhau.2022.02.010

湖南省科学技术厅“三区”科技人才项目(湘科计[2021]35号)。

本校为第一机构

采用平板分离法、平板对峙法从龙牙百合内生菌中筛选获得了具有抑制灰葡萄孢菌活性的98号菌,其抑菌率为74%;对98号菌进行生理生化测定及16S rDNA序列分析,鉴定为唐菖蒲伯克霍尔德菌(Burkholderia gladioli)。通过单因素和正交试验优化该菌株的发酵条件和最适发酵培养基配方,结果表明,pH值为7、培养温度为32 ℃、接种量为发酵液体积的6%、250 mL三角瓶装100 mL牛肉膏蛋白胨液体发酵培养基、180 r/min的摇床培养5 d,其抑菌活性最大;优化后的最适培养基配方为硫酸亚铁0.15 g、玉米粉20 g、牛肉膏15 g,水1 L。

Strain 98 with the activity of inhibiting Botrytis cinerea was screened from the endophytic bacteria of Lilium brownii var.viridulum Baker by plate separation method and plate confrontation method,and the antibacterial rate was 74%.The endophyte strain 98 was identified as Burkholderia gladioli by physiological and biochemical assays and 16S rDNA sequence analysis.The fermentation conditions and the formula of fermentation medium for strain 98 were optimized by single factor and orthogonal tests.The results showed that 100 mL of fermentation me...