期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2024

50

02

50-56

10.13331/j.cnki.jhau.2024.02.008

XCZX-2023141:农业部项目（非规范项目名称） 2020kjc-js170:湖南硒海生物科技有限公司项目

本校为第一机构

以湖南武冈种植的甜型和非甜型青钱柳叶为材料,分离其RNA并构建cDNA文库,开展 2 种类型青钱柳的转录组测定.利用转录组数据结合萜类代谢组及其代谢途径分析,研究青钱柳发育过程中甜味性状形成的分子差异.对甜型与非甜型青钱柳叶片转录组的差异基因GO及KEGG分析,共鉴定出 13 个参与三萜类化合物形成的关键酶基因,在 2 种青钱柳叶中具有显著差异性表达模式.结合萜类代谢检测结果,甜型青钱柳叶中 4 类甜味三萜类化合物的积累量显著高于非甜型青钱柳叶的,说明这些基因的差异表达造成了甜味三萜类化合物在叶片中积累量的不同,应是青钱柳甜味差异性状形成的关键原因.

In this study,leaves of sweet and non-sweet taste Cyclocarya paliurus leaves planted in Wugang,Hunan Province were selected for comparative study to illustrate the mechanism of sweet taste formation.The cDNA libraries were constructed and sequenced.The transcriptome date was analyzed with there terpenoid metabolome and the metabolism pathway.The molecular differences in the formation of sweetness traits of Cyclocarya paliurus were explored.Some 13 key enzyme genes involved in triterpene biosynthesis to have significantly different expression patterns between the two materials by GO and KEGG an...