期刊论文

Riboflavin inhibited ischemia brain damage in rats

中文

中国药理学通报

Chinese Pharmacological Bulletin

1001-1978

2016

32

8

1115-1121

10.3969/j.issn.1001-1978.2016.08.017

国家自然科学基金资助项目(No 81402922)；

本校为其他机构

目的：探讨核黄素对缺血性脑损伤的保护作用及其机制。方法在体实验，采用SD ♂大鼠，随机分为对照组、模型组和核黄素组。核黄素组大鼠腹腔注射核黄素1 mg?kg－1，连续7 d后，模型组和核黄素组大鼠用线栓法制作大脑中动脉栓死（middle cerebral artery occlusion ，MCAO）模型。术后24 h，用2，3，5－氯化三苯基四氮唑（ triphenyl tetrazolium chloride，TTC）检测脑梗死面积、称重法检测脑水肿状况、电镜观察缺血处脑皮层超微结构变化。离体实验采用原代培养的大鼠脑皮层神经元制备氧糖剥夺（ oxygen and glycose deprivation，OGD）模型。用噻唑蓝[3－（4，5－dimethylthiazol－2yl）－2，5...

Aim To investigate the protective effect of riboflavin on ischemia brain damage and the mechanism. Methods The in vivo experiments were processed in male SD rats. Rats were randomly arranged into control group, model group and riboflavin group. The rats in riboflavin group were intraperitoneally injected riboflavin at the dose of 1 mg • kg"1 for seven consecutive days. Then the rats in model and riboflavin groups were carried out middle cerebral artery occlusion (MCAO) operation. After 24 h, all rats were sacri ficed and the brain tissue was d...