期刊论文

Effects of doxorubicin on neuropathic pain in chronic sciatic nerve constriction injury rats and its underly ing mechanism

Yong-Jian, Z.

中文

中国药理学通报

Chinese Pharmacological Bulletin

1001-1978

2018

34

1

33-38

10.3969/j.issn.1001-1978.2018.01.009

国家自然科学基金面上项目(No 81773828,81273600)； 河北省自然科学基金资助项目(No C2011206145)；

本校为其他机构

目的观察阿霉素(DOX)对坐骨神经慢性缩窄性损伤(CCI)模型大鼠的镇痛作用,并从形态学及组织凋亡蛋白的角度对其机制进行分析。方法将SD大鼠随机分为4组:假手术组(Sham) 、CCI模型组(Model) 、假手术+阿霉素5 mg·kg~(-1)组(Sham + DOX) 、CCI模型+阿霉素5 mg·kg~(-1)组(Model + DOX) 。造模成功后,各组采用尾静脉注射的方式给药,Sham组和Model组给予等量生理盐水,检测各组大鼠机械痛阈值和热痛阈值。在行为学检测结束后,即手术后d 15取大鼠右侧L_(4-5) DRG,观察DRG细胞形态、超微结构及DOX的分布情况,采用Western blot法测定DRG组织中Bax、Bcl-2、PKCα、PKCδ及PKCε的蛋白表达。结果静脉注射D...

Aim To observe the analgesic effect of doxorubicin (DOX) on chronic sciatic nerve constric tion injury (CCI) rat model, and analyze the underly ing mechanism from the ultrastructure of sciatic nerve ganglion and the expressions of some apoptotic proteins. Methods A total of 60 SD rats were randomly divided into four groups: Sham operation group (Sham), CCI model group (Model), sham operation + DOX 5 mg. kg⁻¹ group (Sham + DOX), CCI model + DOX5 mg kg⁻¹ group (Model+DOX). DOX was given by caudal vein injection after model establishment. Sham...