期刊论文

Deng, Wangqiu;Li, Taihui

英文

Computational and Structural Biotechnology Journal

2001-0370

2020

18

2081-2094

10.1016/j.csbj.2020.07.014

The final protein solution was diluted with 0.5 M TEAB and then digested with trypsin (Promega, Madison, WI, USA) (enzyme: protein ratio of 1:20) at 37 °C for 2 h. Peptides were labelled using the IBT method as previously described [37]. A mass spectrometric analysis of soluble intracellular proteins was undertaken, as previously described [38]. Raw MS/MS data was queried against the T. guangdongense genome using the Mascot search engine MaxQuant (version 2.3.02), and was deposited in the ProteomeXchange Consortium (identifier number PXD020391). The peptides labeled with IBTs were quantitatively analyzed using the IQuant software [38]. Proteins with a P < 0.05 and a fold change > 1.50 or < 0.67 were defined as differentially expressed proteins (DEPs). These proteins were classified and grouped using the GO and KEGG databases, and a hypergeometric test was used to analyze the GO and KEGG pathway annotations. The interaction network and subcellular location were predicted using the STRING protein interaction database and WoLF PSORT software [39], [40].

本校为其他机构

Tolypocladium guangdongense has a similar metabolite profile to Ophiocordyceps sinensis, a highly regarded fungus used for traditional Chinese medicine with high nutritional and medicinal value. Although the genome sequence of T. guangdongense has been reported, relatively little is known about the regulatory networks for fruiting body development and about the metabolite biosynthesis pathways. In order to address this, an analysis of transcriptome and proteome at differential developmental stages of T. guangdongense was performed. In total, 90...