期刊论文

Regulating elements and expression analysis of PIN3 gene in Brassicaceae family

中文

中国农业大学学报

1007-4333

2017

22

7

24-33

10.11841/j.issn.1007-4333.2017.07.03

湖南省教育厅高校重点实验室开放项目（15K060）；

本校为第一机构

为探明十字花科不同物种PIN3基因的表达调控差异,从拟南芥(Arabidopsis thaliala)、红花荠菜(Capsella rubella)、白菜(Brassica rapa)、油菜(Brassica napus)和甘蓝(Brassica oleracea)等17个已完成基因组测序的十字花科物种基因组数据库中鉴定出18个PIN3同源基因。对这18个同源基因编码序列上游1 500 bp启动子区域顺式作用元件进行预测及比对分析,结果发现,光响应、多种激素响应及胁迫响应等相关元件呈现出较大差异,其中生长素以及向地性响应相关元件具有较高的保守性。克隆普通荠菜PIN3同源基因(CbPIN3)并构建普通荠菜PIN3基因启动子的GUS报告系统CbPIN3 pro::GUS转化拟南芥。与拟南芥基因表...

A total of 18 homologous PIN3 genes were identified in 17 species in Brassicaceae family based on genomic data available including Arabidopsis thaliana,Capsella rubella,Brassica rapa and Brassica oleraceaetc.Gene sequences were subjected to bioinformatics analysis and light-,hormone-response elements and stress-response elements are differently contained in the gene promoter as cis elements.The auxin-response and geotropism response elements are conservative among the 18 homologous genes.The promoter of PIN3 (CbPIN3) from Capsella bursapastoris...