期刊论文

中文

中国农业大学学报

1007-4333

2022

27

8

162-174

10.11841/j.issn.1007-4333.2022.08.15

生猪产业前沿关键科学和技术研究项目（2019xny-js044）；

本校为其他机构

为分析两种脂肪供能条件下3种不同油脂饮食对小鼠腹股沟皮下脂肪沉积及其巨噬细胞分型的影响,90只C57BL/6J小鼠被随机分为6组,分别饲喂低脂猪油(Lar-10%)、低脂菜籽油(Rap-10%)、低脂橄榄油(Oli-10%)、高脂猪油(Lar-30%)、高脂菜籽油(Rap-30%)和高脂橄榄油(Oli-30%),16周后解剖取腹股沟皮下脂肪组织,并对其进行苏木精-伊红染色,活性氧(Reactive oxygen species,ROS)含量检测,M1、M2型巨噬细胞标记物(CD11c和CD206)荧光双染,并通过ELISA检测其含量。结果表明:1)在低脂饮食条件下,三种油脂对小鼠腹股沟皮下脂肪组织重量、脂肪细胞横截面积、腹股沟皮下脂肪ROS含量均无显著影响(P>0.05);2)随着脂肪...

The study explored the effects of three different oil and fat diets on inguinal subcutaneous fat deposition and macrophage typing in mice under two kinds of fat energy supply conditions.A total of 90C57BL/6Jmale mice were randomly divided into 6groups,and fed with low-fat lard(Lar-10%),low-fat rapeseed oil(Rap-10%),low-fat olive oil(Oli-10%),high-fat lard(Lar-30%),high-fat rapeseed oil(Rap-30%),and high-fat olive oil(Oli-30%).After 16weeks,the inguinal subcutaneous adipose tissues were dissected and stained by hematoxylin-eosin staining,reactive oxygen species(ROS)content was detected.M1and M2...