Development of a double-antibody sandwich ELISA for quantification of mutated EPSPS gene expression in rice

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成果类型：

期刊论文

作者：

Luo, Biao;Zhang, Xianwen;Wang, Fang;Wang, Yan;Wu, Wei;...

通讯作者：

Rao, Liqun;Wang, QM

作者机构：

[Wang, Qiming; Luo, Biao; Rao, Liqun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

[Luo, Biao; Zhang, Xianwen; Wang, Fang; Wang, Yan] Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China.

[Zhang, Xianwen] Zhejiang Acad Agr Sci, Key Lab Traceabil Agr Genet Modified Organisms, Minist Agr & Rural Affairs, Hangzhou 310021, Zhejiang, Peoples R China.

[Wu, Wei] Shanghai YouLong Biotech Co Ltd, Shanghai 200063, Peoples R China.

[Lin, Chaoyang] Zhejiang Univ, Inst Insect Sci, Rural Dev Acad, Hangzhou 310063, Zhejiang, Peoples R China.

通讯机构：

[Rao, LQ; Wang, QM ] H

Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

5-Enolpyruvyl-shikimate-3-phosphate synthase (EPSPS);DAS-ELISA;OsmEPSPS detection;Glyphosate-tolerant rice;Glyphosate-tolerant rice

期刊：

Analytical Biochemistry

ISSN：

0003-2697

年：

2025

卷：

696

页码：

115669

DOI：

10.1016/j.ab.2024.115669

基金类别：

This work was supported by the research fund of Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2021C02064-6). CRediT authorship contribution statement Biao Luo: Writing – original draft, Visualization, Validation, Investigation, Data curation. Xianwen Zhang: Validation, Resources, Investigation, acquisition, Data curation. Fang Wang: Visualization, Validation. Yan Wang: Methodology, Formal analysis. Wei Wu: Resources. Chaoyang Lin: Methodology, Formal analysis. Liqun Rao: Writing – review & editing, Supervision, Project administration, Conceptualization. Qiming Wang: Writing – review & editing, Supervision, Conceptualization.

机构署名：

本校为第一且通讯机构

院系归属：

生物科学技术学院

摘要：

Glyphosate resistance is a critically important trait for genetically modified (GM) crops. Mutation of the rice EPSPS gene results in a high level of glyphosate resistance, presenting significant potential for the development of glyphosate-tolerant crops. The resistance of rice to glyphosate is correlated with the expression levels of resistance genes. Therefore, developing a convenient, stable, and sensitive method for quantifying the OsmEPSPS protein is crucial for the development of glyphosate-resistant crops. We developed a double-antibody ...

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Development of a double-antibody sandwich ELISA for quantification of mutated EPSPS gene expression in rice

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