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Development of a double-antibody sandwich ELISA for quantification of mutated EPSPS gene expression in rice

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成果类型:
期刊论文
作者:
Biao Luo;Xianwen Zhang;Fang Wang;Yan Wang;Wei Wu;...
通讯作者:
Liqun Rao
作者机构:
[Biao Luo] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan Province, 410128, China
[Biao Luo] Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang Province, 310021, China
[Xianwen Zhang; Fang Wang; Yan Wang] Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang Province, 310021, China
[Xianwen Zhang] Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang Province, 310021, China
[Wei Wu] Shanghai YouLong Biotech Co., Ltd., Shanghai, 201114, China
通讯机构:
[Liqun Rao] C
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan Province, 410128, China
语种:
英文
关键词:
5-Enolpyruvyl-shikimate-3-phosphate synthase (EPSPS);DAS-ELISA;Glyphosate-tolerant rice;OsmEPSPS detection
期刊:
Analytical Biochemistry
ISSN:
0003-2697
年:
2025
卷:
696
页码:
115669
基金类别:
This work was supported by the research fund of Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2021C02064-6). CRediT authorship contribution statement Biao Luo: Writing – original draft, Visualization, Validation, Investigation, Data curation. Xianwen Zhang: Validation, Resources, Investigation, acquisition, Data curation. Fang Wang: Visualization, Validation. Yan Wang: Methodology, Formal analysis. Wei Wu: Resources. Chaoyang Lin: Methodology, Formal analysis. Liqun Rao: Writing – review & editing, Supervision, Project administration, Conceptualization. Qiming Wang: Writing – review & editing, Supervision, Conceptualization.
机构署名:
本校为第一且通讯机构
院系归属:
生物科学技术学院
摘要:
Glyphosate resistance is a critically important trait for genetically modified (GM) crops. Mutation of the rice EPSPS gene results in a high level of glyphosate resistance, presenting significant potential for the development of glyphosate-tolerant crops. The resistance of rice to glyphosate is correlated with the expression levels of resistance genes. Therefore, developing a convenient, stable, and sensitive method for quantifying the OsmEPSPS protein is crucial for the development of glyphosate-resistant crops. We developed a double-antibody ...

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