Strain Y7 screened with higher activity was used to produce lipase. The optimal culture conditions were determined as follows：1. 5% NaHCO3 and 1.0% glucose used as carbon sources,1.5% peptone used as nitrogen sources ,0.1% MgSO4 · 7H2O in culture medium ,initial pH 9.0 ,incubation at 30 ℃ for 48 h. It found that the strain had selective effect to the natural oils and organic acids, only olive oil had significant stimulation effect, while oleic acid or lactic acid could weaken the production of lipase greatly. Adding 1.0% olive oil to the cul...
