期刊论文

Protective effect of selenium rich oil-tea camellia seed oil on HaCaT cell injuried by hydrogen peroxide

中文

中国油脂

1003-7969

2019

44

1

61-66

10.3969/j.issn.1003-7969.2019.01.014

湖南省教育厅科学研究项目(16B188) 湖南省科技厅重点研发计划(2017NK2173).

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探究油茶籽油对过氧化氢诱导人永生化表皮细胞(HaCaT)氧化损伤的保护作用。以油茶果经物理压榨所得油茶籽油为材料,以体外培养的HaCaT细胞为试验对象,试验分为空白对照组、过氧化氢(H_2O_2)模型损伤组、阳性对照组(V_C)、处理组(油茶籽油5~500 μg/mL预处理),以细胞内细胞存活率(凋亡率)、SOD、GSH-PX、ROS及其Nrf2基因表达水平为综合评价指标,评价油茶籽油的抗氧化性。结果表明:当加入油茶籽油对细胞进行预保护后,质量浓度50~200 μg/mL的油茶籽油对损伤细胞的存活率在70%~82%之间,较H_2O_2模型损伤组的高。50 μg/mL油茶籽油处理组细胞凋亡率为4.49%,较H_2O_2模型损伤组降低了44.77%;细胞内SO...

The protective effect of oil-tea camellia seed oil on the human immortalized epidermal cells (HaCaT) injuried by hydrogen peroxide was studied. The oil-tea camellia seed oil from Camellia oleifera obtained by physical pressing was used as material. HaCaT cells cultured in vitro were used as the experimental objects. The experiment was divided into control group, H_2O_2 model damage group, positive control group (V_C), and treatment group (oil-tea camellia seed oil, 5-500 μg/mL), using cell survival rate(apoptosis rate) in cell, SOD level, GSH-...