期刊论文

中文

中国油脂

1003-7969

2024

49

01

114-119

10.19902/j.cnki.zgyz.1003-7969.220662

21808052:国家自然科学基金 SYL201802002:湖南农业大学双一流建设项目 kq2106049:长沙市杰出创新青年培养计划

本校为第一机构

前列腺素F2α(PGF2α)具有广泛的生理活性,是一种重要的脂质介质.为了实现PGF2α的绿色生物合成,构建了pET30a-TbPGFS、pET30a-MmPGHS、pET30a-GvPGHS 载体并在大肠杆菌BL21(DE3)中分别表达,同时对异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达条件进行了优化以提高重组蛋白在大肠杆菌中的表达量.另外,以最佳诱导表达条件下得到的重组蛋白为催化剂,催化花生四烯酸合成PGF2α.结果表明:MmPGHS蛋白在大肠杆菌中没有表达;GvPGHS蛋白的最佳诱导表达条件为诱导剂IPTG浓度0.2 mmol/L、诱导温度30℃、诱导时间2 h,TbPGFS蛋白的最佳诱导表达条件为诱导剂IPTG浓度0.2 mmol/L、诱导温度30℃、诱导时间6 h;在最佳...

As the important lipid mediators,prostaglandin F2α(PGF2α)performs a wide range of physiological activities.To achieve the green biosynthesis of PGF2α,pET30a-TbPGFS,pET30a-MmPGHS and pET30a-GvPGHS vectors were constructed and expressed in Escherichia coli BL21(DE3),respectively,and the induction expression conditions of isopropyl-β-D-thiogalactopyranoside(IPTG)were optimized to improve the expression of the recombinant protein in E.coli.In addition,the recombinant protein obtained under the optimal conditions were used as a catalyst to catal...