期刊论文

Isolation, Identification and Degradation Characters of a Crude Oil-Degrading Strain ODB01

Lu, X.-Y.

中文

生态与农村环境学报

1673-4831

2017

33

5

466-473

10.11934/j.issn.1673-4831.2017.05.012

2015GK1017:湖南省战略性新兴产业关键共性技术导向类项目 :西藏自治区财政专项

本校为第一且通讯机构

从长庆油田被原油污染土壤中筛选出1株以原油为唯一碳源的菌株ODB01。经菌株生理生化特征及16S rDNA序列分析,鉴定为肠杆菌属(Enterobacter sp.)细菌;运用响应曲面法优化细菌ODB01对原油的降解条件为pH值为8.91,w(NaCl)为1.19%,油菌比为1∶4.12,温度为36.78 ℃,在该条件下细菌ODB01对原油的降解率为34.6%;分别添加w=0.05%土温80(Tween 80)和w=0.05%辛基苯基醚(Triton X-100)后,细菌ODB01对原油降解率分别达到42.5%和46.1%。结果表明,细菌ODB01对原油具有一定的降解能力,有望作为一种微生物修复剂进行开发。

A crude oil-degrading bacteria ODB01 was isolated from crude oil-contaminated soil of Changqing Oilfield, capable of utilizing crude-oil as the sole source of carbon for growth, and identified as Enterobacter sp., based on physiological characteristics and the analysis of 16S rDNA gene sequence. The optimization of crude oil degradation condition was carried out by response surface methodology (Box-Behnken), the results are as follows: pH, 8.91; NaCl concentration, 1.19%; oil-bacteria liquid proportion, 1:4.12; temperature, 36.78℃, and degrada...