Objective To establish an in vitro culture system for Carpoorotus eaulis L.Methods, Using C. edulis L. seeds in ripe fruits as explants, explore the effects of different plant growth regulators on callus induction, adventitious buds differentiation, and rooting in vitro. Results When the media was MS+2,4-D 2.0 mg/L+6-BA 0.2 mg/L, the germination rate was 70%, and callus induction rate was 100%. The optimum media for caespitose buds induction and proliferation was MS+6-BA 2.0 mg/L. Both piantlets rooting rate and survival rate reached 95% whe...