期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2014

40

6

583-588

10.13331/j.cnki.jhau.2014.06.004

2011AA10020901:国家高技术研究发展计划（863计划） SR0373:美国孟德尔生物技术公司合作项目

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以芒(Miscanthus sinensis)的幼穗为材料,通过外植体消毒灭菌、愈伤组织诱导、不定芽分化增殖、生根和驯化移栽,建立芒组织培养的快速繁殖体系。结果表明:芒愈伤组织诱导的最佳培养基是MS+4.0 mg/L 2,4-D+1.0 mg/L 6-BA+0.5 mg/L CuSO_4,愈伤组织诱导频率达99.33%;不定芽分化和增殖的最佳培养基分别为MS+1.0 mg/L 6-BA+0.4 mg/L NAA+0.4 mg/L IAA和MS+ 3.0 mg/L 6-BA +1.5 mg/L PP_(333) +1.0 mg/L CCC,分化率为96%,增殖系数为9.1;生根培养基选用MS+0.5 mg/L IAA+0.5 mg/L NAA+0.5 mg/L CCC+0.5 mg/L PP_(333),生根率达100%;将再生苗移栽至用黄土和营养土配制的基质上,其成活率高达95%,且生长势...

The rapid propagation system was established using the spike of Miscanthus sinensis as material through explants sterilization, adventitious bud differentiation and multiplication, rooting and transplanting acclimation. The results showed that the optimal medium for callus induction was MS＋ 4.0 mg/L 2,4-D ＋ 1.0 mg/L 6-BA ＋ 0.5 mg/L CuSO4, with which the induction frequency reached 99.33%. Best adventitious bud differentiation and multiplying medium was MS＋ 1.0 mg/L 6-BA ＋ 0.4 mg/L NAA ＋ 0.4 mg/L IAA and MS＋ 3.0 mg/L 6-BA ＋ 1.5 mg/L PP33...