期刊论文

Wang, Aibing

英文

PLOS ONE

1932-6203

2018

13

2

e0192641

10.1371/journal.pone.0192641

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31571432, 31270819]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [2015JC3097]; NHLBI/NIH Division of Intramural Research [ZIA HL004228]; NATIONAL HEART, LUNG, AND BLOOD INSTITUTEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Heart Lung & Blood Institute (NHLBI) [ZIAHL004228, ZIAHL004228, ZICHL005907, ZICHL005907, ZICHL005907, ZIAHL004223, ZICHL005907, ZIAHL004228, ZICHL005907, ZIAHL004228, ZICHL005907, ZIAHL004223, ZIAHL004223, ZICHL005907, ZIAHL004228, ZIAHL004223, ZIAHL004228, ZICHL005907, ZIAHL004228, ZICHL005907, ZIAHL004228, ZIAHL004228, ZIAHL004228, ZICHL005907, ZIAHL004228] Funding Source: NIH RePORTER

本校为其他机构

Targeted integration of exogenous genes into so-called safe harbors/friend sites, offers the advantages of expressing normal levels of target genes and preventing potentially adverse effects on endogenous genes. However, the ideal genomic loci for this purpose remain limited. Additionally, due to the inherent and unresolved issues with the current genome editing tools, traditional embryonic stem (ES) cell-based targeted transgenesis technology is still preferred in practical applications. Here, we report that a high and repeatable homologous recombination (HR) frequency (>95%) is achieved when...