期刊论文

中文

湖南大学学报(自然科学版)

1674-2974

2002

29

1

8-12

10.3321/j.issn:1000-2472.2002.01.002

本校为其他机构

取库拉索芦荟(Aloe vera L.)的茎段、叶片作外植体接种于MS附加不同激素的培养基上,诱导出丛生芽、微块茎、愈伤组织,将丛生芽、微块茎、愈伤组织分别用浓度为0.01%,O.02%,O.05%的秋水仙素以不同的方法和时间处理,丛生芽和微块茎能较好地诱出变异体,其中以0.02%的秋水仙素棉球覆盖处理丛生芽48 h诱变效果最好.变异体叶片肥厚、根粗壮.筛选稳定的变异体继代,经染色体数目观察,二倍体植株染色体数目为2n＝2×x＝14,X=7,不同变异体染色体数目不同,其中80%以上的变异体细胞染色体数2n=4×x＝28,x＝7,为同源四倍体.四倍体气孔器明显大于二倍体.四倍体试管苗在MS+BA4mg/1 NAA0.2mg/l可以大量增殖,并于...

The stem segments and leaves of Aloe vera L. were cultured on MS medium with different hormones. The calli,microtubers and clumpy buds were induced, they were treated by various colchicine concentration of 0.01%,0.02%,0.05% during different time by diverse measures, the result indicated that the induced ratio of various plantlets from clumpy buds are the highest when those buds are covered with cotton during 48 hours treated by colchicine concentration of 0.02%.The stable variations were selected to keep on propagating, lots of new buds formed ...