期刊论文

中文

中国畜牧兽医

1671-7236

2010

37

6

63-66

广东省科技计划项目（2009B020307011） 教育部“***和创新团队发展计划”创新团队项目（IRT0723）

本校为第一机构

以5株堆形艾美耳球虫为研究对象,对其线粒体细胞色素c氧化酶第Ⅲ亚基（cox3）的部分基因（pcox3）进行了PCR扩增、测序及序列分析,并与GenBank上已发表的顶复门原虫相关序列进行比较,研究其线粒体cox3基因遗传变异情况。结果每个虫株均获得572bp的目的片段,利用DNAStar软件进行序列比对,5株堆形艾美耳球虫线粒体pcox3序列完全一致,与疟原虫和住白细胞虫相应序列的相似性分别为51%和50%,而与泰勒虫的相似性少于50%。本研究首次报道了堆形艾美耳球虫pcox3序列,结果显示不同来源的堆形艾美耳球虫pcox3序列没有差异,与毒力无关,为进一步研究艾美耳球虫的群体遗传学奠定了基础。

To study genetic variation of mitochondrial cytochrome c oxidase subunit Ⅲ（cox3）gene of Eimeria acervulina,the cox3 was amplified by polymerase chain reaction（PCR）and 572 bp of partial cox3（pcox3）was obtained from five strains of E.acervulina.By sequence comparison and comparative analysis with the cox3 gene of some protozoa of Phylum Apicomplexa avalidable in GenBank,the results showed that there was no difference in the nucleotides of the pcox3 sequences obtained from five strains of E.acervulina,and there were distinct differences in ...