To amplify and analyse the mitochondrial NADH dehydrogenase subunit 1(nad1) gene of Spirometra erinaceieuropaei isolates from Hunan province.The partial nad1(pnad1) was amplified from each Spirometra erinaceieuropaei spargana sample,and pnad1 sequences were aligned using the ClustalX 1.81.Sequence homology analysis was performed using the Megalign program of the software DNAStar version 5.0.The results showed that the lengths of pnad1 were 570 bp.The results provided a foundation for further studies of molecular identificati...