期刊论文

Effect of Chlorpromazine on Apoptosis of Rat Ovary Granulose Cells in Vitro

中文

中国畜牧兽医

1671-7236

2011

12

57-61

国家科技重大专项“重大新药创制”（2009ZX09501-034）；

本校为第一机构

本试验旨在通过氯丙嗪对颗粒细胞凋亡影响的研究,探讨氯丙嗪对雌性大鼠性腺毒性的作用机制.对未成熟的Wistar大鼠卵巢颗粒细胞进行原代培养,用不同浓度的氯丙嗪(0、0.1、1、10 μmol/L)染毒细胞,细胞培养24 h.染毒结束后采用MTT法检测细胞相对活力,荧光染料Hoechest 33258检测颗粒细胞的凋亡变化,RT-PCR检测凋亡调控基因Bax、Bcl-2和P53 mRNA的表达.在本试验所设置的剂量范围内,与对照组比较,氯丙嗪能极显著促进颗粒细胞凋亡(P＜0.01),呈浓度依赖关系；RT-PCR检测则显示氯丙嗪能引起Bcl-2、Bax、P53 mRNA表达水平和Bax mRNA/Bcl-2 mRNA值升高,除低剂量组的Bax mRNA表达水平和Bax mRNA/Bcl-2 mRN...

The aim is to investigate the relationship between female gonad toxicity of chlorpromazine on rat and the apoptosis of granulosa cells.Immature ovarian granulosa cell of Wistar rat was primary cultured,cells were cultured and treated with different concentration chlorpromazine（0,0.1,1,10 μmol/L） for 24 h.After exposured to chlorpromazine 24 h,cell viability was detected by MTT assay,apoptotic cells were identified microscopically by chromatin condensation and nuclear fragmentation with Hoechest 33258,mRNA expressions of Bax,Bcl-2 and P53 wer...