期刊论文

Content Determination of Total Flavonoids from Rosa laevigata Michx.Fruits and Optimization of Cellulase-assisted Extraction

中文

中国畜牧兽医

1671-7236

2016

43

12

3156-3162

10.16431/j.cnki.1671-7236.2016.12.011

地方高校国家级大学生创新创业训练计划项目（12690） 湖南农业大学动物医学院大学生创新性实验计划项目（dycx1511）

本校为第一机构

为了测定金樱子中的总黄酮含量,并对其纤维素酶辅助提取工艺进行优化,本研究以芦丁为对照品,采用双波长分光光度法,确定测定波长500 nm,参比波长580 nm,对金樱子中的总黄酮含量进行测定；以总黄酮得率为评价指标,考察提取温度、乙醇浓度、纤维素酶用量、液料比等4个因素对总黄酮得率的影响,正交优化纤维素酶辅助提取金樱子总黄酮的工艺。结果表明,在19.90~398.00μg 范围内,芦丁的质量与吸光度差值ΔA 呈良好的线性关系(r=0.9981),标准曲线方程为 y=0.0008x-0.0047,该方法下测得其得率为87.94 mg/g,平均加样回收率98.23%,相对标准偏差为8.77%；正交试验优化的纤维素酶法辅助提取金樱子总黄酮的最...

To determine the content of total flavonoids from Rosa laevigata Michx.fruits,and optimize the process of cellulase-assisted extraction,the content of total flavonoids from Rosa laevigata Michx.fruits were determined with a double-wavelength spectrophotometry with de-tection wavelength were 500 nm and 580 nm as the reference,using rutin as a standard material, and the process of cellulase-assisted extraction designed by orthogonal test was optimized with the yield of flavonoids as the evaluation index,and the effects of extraction temperature,c...