期刊论文

中文

中国畜牧兽医

1671-7236

2017

44

9

2809-2815

10.16431/j.cnki.1671-7236.2017.09.037

国家自然科学基金项目（31172377）；

本校为第一机构

试验旨在研究过氧化物酶体增殖物激活受体γ (peroxisome proliferator-activated receptors γ,PPARγ)对猪血管内皮细胞增殖、迁移及小管形成的影响,并探讨PPARγ在猪体外血管生成中的作用.设置PPARγ激动剂组(5、10、15、20 umol/L罗格列酮)、抑制剂组(5、10、15、20 μmol/L T0070907)及对照组,通过iCelligence细胞功能分析、划痕试验和Matrigel基质胶三维培养,构建猪体外血管生成的模型,模拟猪体内血管生成的环境,分别对猪血管内皮细胞的增殖、迁移、小管形成能力进行测定,同时根据NCBI已有的相关序列,应用Primer Premier 5.0软件设计PPARγ基因特异性引物,利用SYBR Green Ⅰ实时荧光定量P...

In order to investigate the effect of peroxisome proliferator-activated receptors γ (PPARγ) on proliferation,migration and tube formation of swine vein endothelial cells,and explore its role in swine placental angiogenesis,PPARγ agonist group (5,10,15 and 20 μmol/L rosiglitazone),inhibitor group (5,10,15 and 20 μmol/L T0070907) and control group were set in this study.Through iCelligence cell function analyzer,scratch test,Matrigel matrix three dimensional cell culture,a model of angiogenesis of swine vein endothelial cells in vitro was es...