期刊论文

Feng, Guangfu;Fang, J

英文

BIOCHEMISTRY

0006-2960

2023

62

14

2128-2136

10.1021/acs.biochem.3c00149

National Natural Science Foundation of China [31672457, 22004035]; China Postdoctoral Science Foundation [2020TQ0094, 2020M682565]; Hunan Provincial Science and Technology Department [2018WK4025, 2020NK2004, 2020ZL2004, 2021JJ40230, 2021JJ30008]; Double First-Class Construction Project of Hunan Agricultural University [SYL201802003, YB2018007, CX20190497]; Science and Technology Innovation Program of Hunan Province [2020RC2054]

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Synthetic red fluorescent protein (RFP) chromophores have emerged as valuable tools for biological imaging and therapeutic applications, but their application in the visualization of endogenous RNA G-quadruplexes (G4s) in living cells has been rarely reported so far. Here, by integrating the group of the excellent G4 dye ThT, we modulate RFP chromophores to create a novel fluorescent probe DEBIT with red emission. DEBIT selectively recognizes the G4 structure with the advantage of strong binding affinity, high selectivity, and excellent photostability. Using DEBIT as a fluorescent indicator, t...