期刊论文

Effects of Auxin on the Carpel Development of Capsella bursa-pastoris

中文

河南农业科学

1004-3268

2018

47

8

88-94

10.15933/j.cnki.1004-3268.2018.08.015

贵州省科技计划项目（黔科合基础[2018]1069）； 贵州理工学院学术新苗培养及探索创新项目（黔科合平台人才[2017]5789-19）；

本校为其他机构

为探讨生长素对荠菜心皮发育的影响,构建了生长素报告系统DR 5∶∶GFP,通过农杆菌介导法获得了转基因荠菜植株。荧光显微镜观察结果显示,荠菜心皮发育早期上端两侧荧光信号较其他部分强。为进一步探讨其分子机制,以RT-PCR方法克隆了荠菜PIN 3基因(CbPIN 3),并做了生物信息学分析。结果表明,CbPIN 3基因编码区全长1 950 bp,可编码649个氨基酸。CbPIN3蛋白存在13个丝氨酸磷酸化位点,包含9个跨膜结构域,与拟南芥PIN3亲缘关系最近。此外,实时荧光定量PCR(qRT-PCR)方法检测荠菜CbPIN 3组织表达特点,发现CbPIN 3基因在荠菜多种组织中均表达,在茎中表达量最高,果荚中表达量最低。

To probe the effects of auxin on the carpel development of Capsella bursa-pastoris,auxin reporting system DR5∶ ∶ GFP was constructed,and then transgenic Capsella bursa-pastoris seedling was obtained via Agrobacterium-mediated transformation. Results demonstrated that the fluorescence signals were stronger in both upper sides than middle and base sides of the carpel. PIN3(CbPIN3) gene coding sequences were cloned by RT-PCR to further explore the molecular mechanism. Sequence analysis showed that the length of the CbPIN3 gene cDNA was 1 950 bp ...