SummaryThe robust cell wall structure of Haematococcus pluvialis (H.pluvialis) consists of polysaccharides and tough non‐hydrolysable sporopollenins, which makes it difficult to extract superpotent antioxidant, astaxanthin from these cells. Therefore, breakdown of cell wall is a key step in the overall process of astaxanthin recovery. In this study, the mechanism of three well‐established chemical techniques for cell disruption of H.pluvialis cysts [ionic liquids (IL), hydrochloric acid (HCl) and multiple enzymes (multi‐enzyme, ME)] on decon...