期刊论文

Li, Y.

中文

色谱

1000-8713

2007

25

5

719-722

10.3321/j.issn:1000-8713.2007.05.020

本校为第一且通讯机构

建立了以1-氟-2,4-二硝基苯基-5-L-丙氨酰胺(FDAA)为手性衍生试剂、高效液相色谱拆分茶氨酸对映体的方法.采用的色谱条件为:Kromasil C18色谱柱;三乙胺-磷酸缓冲液和乙腈为流动相,梯度洗脱,流速1.0 mL/min;检测波长为340 nm;柱温为35 ℃.L-茶氨酸的进样量在1.732×10~(-3)～2.077 μg范围内峰面积与进样量之间的线性关系良好,加标回收率为97.3%～102.0% ,检测限为4.973×10~(-4) μg,定量限为1.223×10~(-3) μg.D-茶氨酸的进样量在1.696×10~(-3)～2.044 μg范围内峰面积与进样量之间的线性关系良好,加标回收率为97.2%～103.2% ,检测限为5.871×10~(-4) μg,定量限为1.236×10~(-3) μg.该方法...

L-Theanine is a unique non-protein amino acid in tea, and shows many physiological functions. To directly extract L-theanine from tea is a lengthy and very costly process. Although the chemical synthesis of theanines is simple, the product is unfortunately a mixture of DL-enantiomers. A chiral derivatization method was developed for the separation and quantification of theanine enantiomers by reversed-phase high performance liquid chromatography ( RP-HPLC). 1-Fluoro-2, 4-dinitrophenyl-5-L-alanine amide (FDAA) was used as the chiral reagent. Thi...