期刊论文

Gong, D.

中文

色谱

1000-8713

2011

29

11

1098-1102

10.3724/SP.J.1123.2011.01098

农业部农药残留试验项目（No.2010P0025）；

本校为第一且通讯机构

建立了高效液相色谱(HPLC)测定水稻和稻田中氯硝柳胺乙醇胺盐残留量的分析方法。稻田水和稻秆样品中的氯硝柳胺乙醇胺盐残留用碱性乙酸乙酯直接提取;而稻田土壤、糙米和谷壳样品则先经碱性乙醇提取,再用乙酸乙酯进行萃取。萃取物经弗罗里硅土柱净化后,经Welchrom C18柱分离,采用紫外检测器检测,外标法定量。在0.01~10.00mg/L范围内,氯硝柳胺乙醇胺盐的峰面积与其质量浓度间呈良好的线性关系,相关系数为0.999 8。在稻田水、土壤、稻秆、糙米和谷壳中添加0.01~5.00mg/kg的氯硝柳胺乙醇胺盐,其平均回收率为93.47% ~100.9%,相对标准偏差为1.46% ~5.82%,符合农药残留量分析与检测的技术要求。该方法简...

A high performance liquid chromatographic (HPLC) method was established for the analysis of niclosamide ethanolamine residue in rice and paddy field. The paddy water and plant were extracted with alkaline ethylacetate, while the paddy soil, rice husk and unpolished rice were first extracted with alkaline ethanol, and then with ethylacetate. The extracts were then cleaned-up by a Florisil column and detected by high performance liquid chromatography with an ultraviolet detector (UVD) on a Welchrom C18 column (250 mm×4.6 mm, 5 μm). The calibrat...