版权说明 操作指南
首页 > 成果 > 详情

反相高效液相色谱法同时测定咖啡豆中的6种酚酸类化合物

认领
导出
Link by 中国知网学术期刊 Link by 万方学术期刊
反馈
分享
QQ微信 微博
成果类型:
期刊论文
作者:
龙文静;张盛;袁玲;李银花;刘仲华
通讯作者:
Zhang, S.
作者机构:
[龙文静] National Res. Center of Engineering Tech. for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China
[袁玲] Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China
Department of Botanical Resources Engineering, College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China
[李银花; 刘仲华] National Res. Center of Engineering Tech. for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China, Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China
[张盛] National Res. Center of Engineering Tech. for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China, Department of Botanical Resources Engineering, College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China
通讯机构:
[Zhang, S.] N
National Res. Center of Engineering Tech. for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China
语种:
中文
关键词:
反相高效液相色谱法;酚酸类化合物;咖啡豆
关键词(英文):
Coffee bean;Phenolic acids;Reversed-phase high performance liquid chromatography (RP-HPLC)
期刊:
色谱
ISSN:
1000-8713
年:
2011
卷:
29
期:
5
页码:
439-442
基金类别:
20091101:教育部***和创新团队发展计划项目
机构署名:
本校为第一机构
院系归属:
园艺园林学院
摘要:
建立了同时测定咖啡豆中6种酚酸类化合物(咖啡酸、3-咖啡酰奎尼酸、4-咖啡酰奎尼酸、5-咖啡酰奎尼酸、 3,5-二咖啡酰奎尼酸、4,5-二咖啡酰奎尼酸)的反相高效液相色谱测定方法。采用Kro masil C_(18)柱(200 mm×4.6 mm,5μm),以乙腈和0.1%甲酸水溶液为流动相进行梯度洗脱,二极管阵列检测器检测,45 min内可对6种目标物进行同时检测,且各化合物都能达到基线分离。经测定,样品中6种酚酸类化合物的加标回收率为90.76%~ 104.73%,相对标准偏差为0.7%~3.9%。该法简便、快速、灵敏度高,适用于咖啡豆中6种酚酸类化合物的同时分析以及咖啡豆原料与制品的质量控制和综合评价。
摘要(英文):
A method of reversed-phase high performance liquid chromatography coupletk with diode array detection (HPLC-DAD) was established for the simultaneous determination of 6 phenolic acids in green coffee beans. These phenolic acids are caffeic acidr 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3,5-0-dicaffeolquinic acid and Ay 5-0-dicaffeoylquinic acid. The separation was achieved using a Kromasil C18 column (200 mm x4. 6 mm, 5 μm) under the gradient elution with the mobile phases of acetonitrile and 0. 1% (v/v) formi...

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com