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Arabidopsis thaliana Plants Engineered to Produce Astaxanthin Show Enhanced Oxidative Stress Tolerance and Bacterial Pathogen Resistance

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成果类型:
期刊论文
作者:
Yu, Lujun;Chen, Qnfang;Peng, Yujun;Xie, Lijuan;Liu, Di;...
通讯作者:
Li, Juan;Huang, Junchao
作者机构:
[Xiao, Shi; Han, Muqian; Peng, Yujun; Chen, Qnfang; Liu, Di; Li, Juan; Xie, Lijuan; Yu, Lujun] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol & Guangdong Prov Key Lab, Guangzhou 510275, Guangdong, Peoples R China.
[Han, Muqian; Li, Juan] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
[Chen, Feng] Shenzhen Univ, Inst Adv Study, Shenzhen 518000, Guangdong, Peoples R China.
[Huang, Junchao] Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China.
通讯机构:
[Li, Juan] S
[Li, Juan] H
[Huang, Junchao] C
Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol & Guangdong Prov Key Lab, Guangzhou 510275, Guangdong, Peoples R China.
Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Biosynthesis;Genes;Metabolism;Oxidation;Oxidative stress;Pathogens;Pigments;Astaxanthin;Biosynthetic pathway;Biotic and abiotic stress;Carotenoid;Chlamydomonas reinhardtii;Heterologous expression;Pseudomonas syringae;Transcriptome analysis;Plants (botany);abscisic acid;astaxanthin;phytohormone;xanthophyll;Arabidopsis;biosynthesis;chemistry;gene expression regulation;genetics;metabolism;microbiology;oxidative stress;physiology;plant disease;Pseudomonas syringae;transgenic plant;Abscisic Acid;Arabidopsis;Gene Expression Regulation, Plant;Oxidative Stress;Plant Diseases;Plant Growth Regulators;Plants, Genetically Modified;Pseudomonas syringae;Xanthophylls
期刊:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN:
0021-8561
年:
2019
卷:
67
期:
45
页码:
12590-12598
基金类别:
This work was supported by the National Natural Science Foundation of China (Projects 31725004 31670276, 31461143001, and 31870237), the Pearl River S&T Nova Program of Guangzhou (201610010070), and the Major Project of China on New Varieties of GMO Cultivation (2018zx08011-01B to J.L.).*%blankline%* The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jafc.9b04589 . Figure S1, Analysis of DEGs in the leaves and siliques; Figure S2, Comparison of microarray and qRT-PCR data; Figure S3, Expression profiling of transcription factors in transgenic Arabidopsis expressing CrBKT ; Figure S4, P5CS1 expression in the leaf and silique tissues of WT and CR5 plants; Figure S5, BKT-overexpressing transgenic plants were more tolerant to MV stress; Table S1, DEGs in astaxanthin pathway affected by CrBKT ; Table S2, DEGs of phytohormone-related pathway affected by CrBKT ; Table S3, DEGs of transcription factor affected by CrBKT ; and Table S4, List of primers used for qRT-PCR experiment ( PDF ) This work was supported by the National Natural Science Foundation of China (Projects 31725004, 31670276, 31461143001, and 31870237), the Pearl River S&T Nova Program of Guangzhou (201610010070), and the Major Project of China on New Varieties of GMO Cultivation (2018zx08011-01B to J.L.). The authors declare no competing financial interest.
机构署名:
本校为通讯机构
院系归属:
农学院
摘要:
Carotenoids play key roles in photosynthesis and photoprotection. Few multicellular plants produce the ketocarotenoid astaxanthin, a strong antioxidant; however, Arabidopsis thaliana lines overexpressing the Chlamydomonas reinhardtii β-carotene ketolase (CrBKT) accumulated high amounts of astaxanthin in the leaves. In this study, we investigated the changed regulation of key metabolic pathways and the tolerance of the engineered plants to biotic and abiotic stresses resulting from the heterologous expression of CrBKT. Transcriptome analysis id...

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