期刊论文

Wang, Xian-Chun

英文

生物化学与生物物理学报

1672-9145

2000

32

3

275-280

国家 8 63高技术发展计划资助!（ 10 3 13 0 1 0 6）&&；

本校为其他机构

The functional amino acid sequence and its neighbouring fragments in the molecule of Amaranth α-amylase inhibitor isolated from seeds of the Mexican crop plant Amaranthus hypochondriacus were transferred, by solid phase chemical synthesis, into N-terminal region of the huwentoxin-I (HWTX-I). The synthetic chimera polypeptide was confirmed by Edman degradation and MALDI-TOF mass spectroscopy. The formation of three disulfide bonds and special conformation of the synthetic chimera was induced by the addition of glutathione. Renatured chimera pol...

运用固相化学合成的方法,将墨西哥苋属植物(Amaranthus hypochondriacus)#alpha#-淀粉酶抑制剂(AAI)分子中已知的活性位点序列及可能的相关序列嵌入虎纹捕鸟蛛毒素-I(HWTX-I)的非活性位点区,并替代相应的序列,构建同时携带两种活性位点的多肽嵌合体。构建的嵌合体能在pH5.5和浓度为9.5×10~(-5) mol/L的条件下,对蜚蠊(Periplaneta americana)消化道的#alpha#-淀粉酶具有明显的抑制活性。同时,利用体外小白鼠膈神经-膈肌标本的神经肌肉接头传递的阻断实验证明,嵌合体还能显示36%的天然虎纹捕鸟蛛毒素-I的神经毒活性。