期刊论文

中文

微生物学报

0001-6209

2022

62

10

3801-3812

10.13343/j.cnki.wsxb.20220085

湖南省教育厅科研基金重点项目（20A257）～～；

本校为第一机构

【目的】蛋白-O-岩藻糖基转移酶1 (protein O-fucosyltransferase 1, POFUT1)是催化蛋白质O-岩藻糖基化的关键酶,在动物和人体内被证明调控一系列的生理病理过程,然而POFUT1基因在果生炭疽菌乃至真菌中还未见报道。本研究旨在克隆果生炭疽菌中CfPOFUT1基因,并分析其生物学功能。【方法】利用RT-PCR技术扩增CfPOFUT1的基因并进行生物信息学分析,构建了CfPOFUT1基因的沉默和过表达载体,通过PEG介导法将载体导入原生质体中获得CfPOFUT1基因的沉默和过表达突变体。测定了野生型菌株、CfPOFUT1沉默菌株和过表达菌株在PDA上的菌丝生长、分生孢子产生、萌发与附着胞形成、胁迫应答和致病力、杀菌剂敏感...

[Objective] Protein O-fucosyltransferase 1(POFUT1),a key enzyme catalyzing protein O-fucosylation,has been verified to regulate a series of physiological and pathological processes in animals and humans.However,POFUT1 gene in Colletotrichum fructicola or even fungi has not been reported.This study aims to clone the CfPOFUT1 gene from C.fructicola and analyze its biological functions.[Methods] RT-PCR was employed to amplify the CfPOFUT1 gene and then the bioinformation of the gene was analyzed.We constructed the CfPOFUT1 silencing and overexpression vectors,which were then transformed into the ...