The extracting methods of β-carotene produced from Rhodotorular Glutinis fermentation were studied,which mainly included cell-break methods,different extracting solvent,extracting time,the ratio of materials to solvent and extracting temperature.The results showed that optimal conditions were obtained with ultrasonic as cell-break method and acetone as solvent.And correspondingly,the optimal refining conditions were as follows: acetone solvent volume to the 1g material was 40ml/g,extracting temperat...