In order to reduce cost of Bacillus natto production, using brewer＇s spent grain ras fermentation matrix, with only addition of glucose and sodium chloride, fermentation conditions for Bacillus natto yield were optimized by response surface methodology. Factors of rotation speed, fermen- tation time and initial pH value, which were screened out from nine fermentation factors affected Bacillus natto yield, were used as significant factors in Plackett-Burman experimental design. After determination of optimal range by the steepest experiment, th...
