期刊论文

中文

中国酿造

0254-5071

2015

34

3

62-65

10.11882/j.issn.0254-5071.2015.03.014

(2012RS4042):湖南省科技计划博士后专项资金 (CXS20113B298):湖南省研究生科创资金

本校为第一机构

利用乙醇-CMC-Na平板初筛,经摇瓶发酵复筛,从谷酒成熟酒醪中筛选获得1株耐酒精纤维素酶活力较高的细菌Lys-1249。经生理生化测定、形态观察及16S r DNA基因序列的同源性分析,将其鉴定为枯草芽孢杆菌（Bacillus subtilis）。经正交试验优化该菌株的产酶条件,表明该菌株在麸皮2.0%,蛋白胨0.6%,K2HPO40.1%,Mg SO4·7H2O 0.05%,Fe SO4·7H2O 0.02%,Mn SO40.01%,培养基初始p H值为7.0,培养温度37℃,发酵周期48 h的条件下,其耐酒精纤维素酶活力达到158.54 U/m L。该菌株在6.0%vol乙醇培养基中生长良好,活性可保持65.95%。

An ethanol-tolerant strain Lys-1249 with cellulase activity was screened from the mature fermented grain mash by ethanol-CMC-Na plate preliminary screening and shake flask fermentation affination. Strain Lys-1249 was identified as Bacillus subtilis according to morphological observation, physiological and biochemical determination and 16S rDNA sequence analysis. The cellulose-producing condition was optimized by orthogonal experiment, and results showed that the ethanol-tolerant cellulase activity reached 158.54 U/ml when strain Lys-1249 was cu...